Application

Anti-PACSIN1 Antibody, N-terminus detects level of PACSIN1 & has been published & validated for use in WB, IH(P).

General description

PACSINs, also known as syndapins, are a protein family consisting of three members that have been implicated in synaptic vesicular membrane trafficking and regulation of dynamin-mediated endocytotic processes (Cuajungco, 2006). It is presumed that all isoforms bind Dynamin, Synaptojanin 1 and N-WASP through their Src homology 3 domains. While PACSIN1 appears to be restricted to neural tissues, two PACSINs are more widespread with PACSIN2 being ubiquitously expressed and PACSIN3 present mainly in skeletal muscle, heart and lung (Modregger, 2001). PACSIN1 has been implicated as playing a central role in synaptic vesicle recycling (Kessels, 2004). Expected molecular weight is 51 kDa.

Immunogen

KLH conjugated synthetic peptide selected from the N-terminal region of human PACSIN1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

PBS containing 0.09% NaN3.

Format: Purified

Quality

Evaluated on a representative lot by Western blot on mouse brain lysate using a 1:100 - 1:500 dilution of Anti-PACSIN1.

Specificity

The antibody recognizes human PACSIN1.

Reactivity with other species has not been tested.

Storage and Stability

Maintain at 2-8°C for up to 6 months from date of receipt. -20°C is recommended for long term storage.

Target description

~57 kDa